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当前位置:首页 > 产品中心 > 培养相关试剂 > Trlzol > TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号

TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号
名称 TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号
型号
报价
特点 ​TRIZOL试剂是直接从细胞或组织中提取总RNA的试剂。它在破碎和溶解细胞时能保持RNA的完整性。加入后离心,样品分成水样层和有机层。RNA存在于水样层中。收集上面的的TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号15596-018}
  • 详细内容
品牌:Invitrogen
货号:15596-018
数量:大量
供应商:invitrogen
规格:100 UG/100ml/200ml
规格:100 UG产品价格:¥1100
规格:100ml产品价格:¥1100
规格:200ml产品价格:¥2100

 

 

 

TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号15596-018

Description
TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

Key Features of TRIzol® Reagent:

• Isolate RNA, DNA, and protein from the same sample
• Superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria

Reliably Purify RNA from Multiple Sample Volumes and Sources
TRIzol® Reagent performs well with small quantities of tissue (50-100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin.


 

TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号15596-018


 
货号名称规格
13778-075Lipofectamine RNAiMAX0.75ml
13778-150Lipofectamine RNAiMAX1.5ml
10296-010Trizol ®  LS Reagent100ml
10296-028Trizol ®  LS Reagent200ml
18374-0585' RACE System for Rapid Amplification of cDNA Ends, Version 2.010 reactions
18373-0193' RACE System for Rapid Amplification of cDNA Ends20 reactions
18064-014SuperScript® II Reverse Transcriptase10,000U
18080-044SuperScript® III Reverse Transcriptase10,000U
18418-012Oligo(dT)12-18 Primer25ug
18418-020Oligo(dT)20 Primer50ul(50uM)
10777-019RNaseOUTTM Ribonuclease Inhibitor5,000U
18080-051SuperScript® III First-Strand50 rxn
11668-027Lipofectamine TM 2000 Reagent0.75ml
11668-0191.5ml
15596-026TRlzol ®Reagent100ml
15596-018TRlzol ®Reagent200ml
11789020BP CLONASE II ENZYME MIX20 REACTIONS
11791020LR CLONASE II ENZYME MIX20 REACTIONS
28025-013M-MLV Reverse Transcriptase40,000U
17504-044B-27(黄颜色)10ml
12587-010B-27(黄颜色)不含维生素A10ml
17502-048N-2  (粉红颜色)5ml

TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号15596-018

 

 

TRLZOLReagent (Ambion®) 总RNA提取试剂,invitrogen货号15596-018

 

配制方法

在2000ml的烧杯中加入以下物质,混合均匀:

500ml重蒸苯酚;

250g异硫氰酸胍;

293mL蒸馏水;

⒘6mL0.75M柠檬酸钠溶液(pH≥7)

26.4mL10%sarcosy(十二烷基肌氨酸钠)溶液

50mL2MNaAc溶液(pH≥4)

 

 

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